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SARS-CoV-2 Spike RBD Nanobody

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产品详情有关产品

SARS-CoV-2 Spike RBD Nanobody

货号：

HYK-200093
规格：
图片：
The Binding Activity of SARS-CoV-2 Spike RBD Nanobody with SARS-CoV-2-S1-RBD
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD () at 2 μg/ml can bind SARS-CoV-2 Spike RBD Nanobody, the EC50 is 0.8674 ng/ml.

In the Colloidal Gold Immunochromatography Assay
detection system, the background of antibody (CSB-RA33245A2GMY) is clean, the detection limit can be as low as 25ng/ml (1.75ng/0.07ml), and the sensitivity is very good.

Binding signal of SARS-CoV-2-S1-RBD () and ACE2 protein-HRP conjugate () was inhibited by SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with the ICC50 is 1.296 nM.

Binding signal of SARS-CoV-2-S1-RBD () and ACE2 protein-HRP conjugate () was inhibited by SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with the IC50 is 0.1074 μg/ml.

SARS-CoV-2 Spike protein RBD His/Sumostar Tag () captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with an affinity constant of 28.2nM as detected by LSPR Assay.

产品详情

产品描述：

This SARS-CoV-2 S1-RBD (Spike Glycoprotein S1 receptor-binding domain) antibody is a recombinant monoclonal antibody (also a Nanobody) generated through the expression of a DNA sequence inserting a human IgG1 Fc domain at the C-terminus, in human embryonic kidney 293 cells (HEK293). The DNA sequence encodes the SARS-CoV-2 spike RBD. The antibody is purified by protein G in vitro. It has been validated with high reactivity towards SARS-CoV-2 S1-RBD by a functional ELISA and good sensitivity for human SARS-CoV-2 spike glycoprotein (S protein) via the Colloidal Gold Immunochromatography Assay (GICA).
It is also validated in Neutralizing and LSPR. In neu assay, the binding signal of the SARS-CoV-2 S1 RBD antibody was inhibited by ACE2 protein-HRP conjugated inhibitor, with a 0.1074 μg/ml IC50. In LSPR assay, the SARS-CoV-2 S1 RBD antibody showed a high affinity with SARS-CoV-2 Spike protein RBD (affinity constant: 28.2nM).
Specifically binding and recognizing the RBD of the SARS-CoV-2 spike glycoprotein, the SARS-CoV-2 S1 RBD antibody can react with samples infected with human coronavirus SARS-CoV-2. But it does not respond to MERS or SARS-CoV spike protein. Akin to other nanobodies, this recombinant nanobody is small and stable, which allows for its reaching to hidden epitopes such as crevices of target proteins.
Uniprot No.：

P0DTC2
别号：

Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov S1 Antibody;Anti-ncov spike Antibody; Anti-novel coronavirus RBD Antibody; Anti-novel coronavirus S1 Antibody; Anti-novel coronavirus spike Antibody; Anti-RBD Antibody; Anti-S1 Antibody; Anti-Spike RBD Antibody; E2 Antibody; E2 glycoprotein Antibody; Human coronavirus spike glycoprotein Antibody; S Antibody; SARS-CoV-2 S1 RBD Antibody; S glycoprotein Antibody; Spike glycoprotein Antibody
反映种属：

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
免疫原：

Recombinant Human Novel Coronavirus Spike glycoprotein(S) (319-541aa) (CSB-YP3324GMY1 and CSB-MP3324GMY1b1)
免疫原种属：

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
象征方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

VHH fusion with human IgG1 Fc
纯化方式：

Affinity-chromatography
克隆号：

A1
浓度：

It differs from different batches. Please contact us to confirm it.
保留缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
产品提供大局：

Liquid
利用领域：

ELISA, GICA, Neutralising

推荐稀释比：

Application	Recommended Dilution
ELISA	1:10000-1:100000
GICA	1:10000-1:40000
Neutralising	1:100-1:10000

Protocols：

ELISA Protocol
贮存前提：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.