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细胞色素P450(cytochromeP450或CYP450,简称CYP450)代表着一个很大的可自身氧化的亚铁血红素蛋白家族,,,,,,属于单氧酶的一类,,,,,,因其在450纳米有特异吸收峰而得名。。。。。。它参加内源性物质和蕴含药物、环境化合物在内的表源性物质的代谢。。。。。。凭据氨基酸序列的同源水平,,,,,,其成员又顺次分为家族、亚家族和酶个别三级。。。。。。细胞色素P450酶系统可缩写为CYP,,,,,,其中家族以阿拉伯数字暗示,,,,,,亚家族以大写英文字母暗示,,,,,,酶个别以阿拉伯数字暗示,,,,,,如CYP2D6、CYP2C19、CYP3A4等。。。。。。
钻研意思:
细胞中,,,,,,细胞色素P450重要散布在内质网和线粒体内膜上,,,,,,作为一种结尾加氧酶,,,,,,参加了生物体内的甾醇类激素合成等过程。。。。。。近年来,,,,,,对细胞色素P450的结构、职能出格是对其在药物代谢中的作用的钻研有了较大的进展。。。。。。钻研批注细胞色素P450是药物代谢过程中的关键酶,,,,,,并且对细胞因子和体温调节都有沉要影响。。。。。。
有关文件:
【文件标题】Enantioselective toxicity and oxidative stress effects of acetochlor on earthworms (Eisenia fetida) by mediating the signaling pathway
【作者】Yalei Liu, Kuan Fang, Xiaolian Zhang,et.al
【作者单元】中国农业科学院烟草钻研所(Tobacco Research Institute of Chinese Academy of Agricultural Sciences)
【文件中引用产品】
蚯蚓8-羟基脱氧鸟苷(8-OHdG)ELISA试剂盒
蚯蚓细胞色素P450(CYP450)ELISA试剂盒
蚯蚓羟基自由基(OH)ELISA检测试剂盒
【关键词】Acetochlor,Enantiomer,Earthworm,Toxicity,Transcriptomic
【DOI】doi.org/10.1016/j.scitotenv.2020.142630
【影响因子(IF)】8.6
【出版期刊】《Science of the Total Environment》
【产品原文引用】
The OH content, 8-OhdG content, and CYP450 content were determined according to the method of Zhang et al. (2020) using a kit obtained from the ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The OH content, 8-OHdG content, and activity of P450 in earthworms were detected by the double antibody sandwich method. The OH in earthworms combined with the antibodies of OH marked by HRP (Horseradish Peroxidase). Then, 3,3′,5,5′-tetramethylbenzidine (TMB) was added to produce a chromogenic reaction with the compound of antibody-antigen-antibody labeled by the enzyme. The absorbance of the reaction solution was recorded at 450 nm. The detection principle and method of 8-OhdG and CYP450 are the same as those of OH.
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